22  IGHV3-35

22.1 Allele appearnce

The group of IGHV3-35* includes 1 functional alleles within it.

This allele group is unique as two alleles are indicated to IGHV3-35 but only one of them is functional IGHV3-35*02, while the other (IGHV3-35*01) is an ORF allele

For each allele we counted the number of appearances across the population, any appearance was considered valid.

22.2 Group alignment

Based on the viewd alleles, we calculated the distance between the germline sequences.

22.3 Sequence depth

To examine the potential cutoff we observed the sequence depth for each allele

22.4 Absolute cutoff

We set an initial cutoff of \(0.01\%\) to determine the potential genotype priors. For this cutoff we examined the zygousity state, such as homozygousity, heterozygousity and so on.

22.4.1 Allele specific cutoff

Observing the alleles frequencies we adapted the following thresholds